•In the field of AI Ethics, one of the main current challenges is to transfer high-level concepts into concepts that can be applied in practice.
•This article presents the design and validation of the content of an ethical checklist (the Canada Protocol) for the use of AI in the field of mental health and suicide prevention.
•The aim of this checklist is to create a bridge between three separated fields: Mental Health, Ethics and Computer Sciences.
•This checklist is intended to help AI developers, researchers and entrepreneurs identify the ethical challenges associated with the use of AI in mental health, and potentially prevent them.
•The Protocol comprises 38 items split into five categories: “Description of the Autonomous Intelligent System” (n = 8), “Privacy and Transparency” (n = 8), “Security” (n = 6), “Health-Related Risks” (n = 8), “Biases” (n = 8).